attune flow cytometer - An Overview
attune flow cytometer - An Overview
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Data-enriched characterization of mobile populations. Morphological info from visuals can insert on the richness of flow cytometry knowledge. Such as, the figure displays an otherwise regular apoptosis assay utilizing Annexin V and PI, including cell imaging to characterize cells in each populace to expose morphologically distinct attributes. These insights could not are already obtained from multiplex staining by itself.
As samples are obtained within the Attune CytPix Flow Cytometer, the superior-velocity brightfield digicam captures and merchants visuals of detected functions at a price nearly six,000 pictures for every second, based on the flow rate and impression dimensions. For increased overall flexibility, Attune Cytometric Software allows you to alter the picture seize frequency as wanted and select a particular gate for impression capture, if required.
(B) RAW 264.7 macrophages are frequently annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The volume of singlets accessible for Assessment improves as soon as the person trains and employs a more precise product.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a higher-speed brightfield camera for simultaneous substantial throughput flow cytometry and large resolution brightfield imaging.
*Pricing is indicative only and is particularly issue to change. Please Speak to your Agilent agent with any issues. The knowledge established forth herein is not a proposal or even a dedication, both of that happen to be topic to credit approval, terms and conditions, and availability with Agilent. Agilent Economical Methods is accessible in picked countries.
Also, it truly is illuminating to determine the number of doublets slip as a result of common singlet gates, and great to validate the standing of unanticipated double-beneficial situations in my Investigation."
Pair your Attune flow cytometer having an autosampler for elevated workflow effectiveness, and increase robotic integration for maximum throughput.
Our researchers will design and style panels for you as many times you will need—free of charge, no merchandise obtain necessary.
Early signs of this contamination had to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also helps you to use morphological features to find out interesting subpopulations that will not be evident from flow cytometry information by itself.
Buyers can perspective occasion photographs in true-time to verify sample high-quality, optimize protocols, and record the morphology of each mobile populace determined for Investigation. Imaging capture also Rewards from acoustic concentrating, which helps you to position the cells making sure that a sharp, centered graphic is acquired.
Sound waves empower exact cell alignment, causing Fantastic details top quality and ~10X quicker more info sample flow charges.
Software Observe: Automatic image analysis cuts down person-to-person variability in flow cytometry gating techniques
Leverage the power of Sophisticated engineering and innovation to accelerate your discoveries. Rely on in the Attune Flow Cytometer household to deliver the trustworthiness, speed, and precision that established your work aside.
Flexible payment system from Agilent Monetary Options permit you to receive the critical instrumentation you would like for rapid job, or to mature your lab.
At the guts of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technology, which utilizes sound waves to meticulously align cells within the flow mobile. This revolutionary method surpasses traditional hydrodynamic methods, leading to faster processing speeds, Outstanding info high quality, and greater sample throughput.
Since the mobile line experienced Beforehand been analyzed around the Attune CytPix Flow Cytometer, the scientists went back to the photographs and have been in the position to document the microbial infection no less than five times earlier.
The Attune CytPix Flow Cytometer enables consumers to visualise samples during acquisition, validate the precision of gating methods, and support final results with responsible documentation for publication functions.
What seemed to be a homogeneous population is really additional diverse—an insight that needs to be regarded as when interpreting outcomes.